Preclinical and clinical studies with withanolides: Therapeutic effects, molecular signatures and biomarkers
One of the hallmarks of ALS is the presence of abnormal clumps inside motor neurons that contain various substances which include, in the majority of cases, something called TAR DNA–binding protein 43 (TDP-43). As a result, understanding the mechanisms by which TDP-43 may influence the disease may have a tremendous impact on our ability to treat ALS. Previous research by Dr. Jean-Pierre Julien and his team showed that TDP-43 interacted with something called nuclear factor- κB (NF-κB), which is master regulator of inflammation, a process that has been implicated in the disease mechanism of ALS. Further work revealed that treatment of ALS model mice in the laboratory with an NF-κB inhibitor called Withaferin A, reduced disease symptoms and neuroinflammation. Furthermore, the plant Withania somnifera (Ashwagandha), from which Withaferin A is derived, also had positive effects in ALS model mice when fed to them. As a result, Dr. Julien started collaboration with ImStar Therapeutics, Inc. to create new drugs that mimic Withaferin A, but with enhanced characteristics to be used for treatment of ALS. This work has resulted in a compound called IMS-088 and this Hudson Grant will fund the preclinical (laboratory) studies to investigate its use as a possible ALS therapy. Using unique ALS mouse models that are termed RiboTag, which will allow instant monitoring of specific cells that are important to the disease as the symptoms progress, Dr. Julien’s team will determine not only if IMS-088 works to slow down the progression of the disease, but will also determine if there are specific biological markers (biomarkers) that can be used to monitor the effectiveness of substance in humans should it succeed in reaching clinical trial. One of the targets they will monitor in the mice is specific blood cells. If IMS-088 treatment effectiveness can be detected, it is possible that a simple blood test might yield all of the information needed to determine if the drug is doing its proper anti-inflammatory job in humans during clinical trial.
Jean-Pierre Julien , Université Laval
Jasna Kriz, Université Laval
Angela Genge, McGill University
Partners and Donors
ALS Society of Canada